Line probe assay test in new cases of tuberculosis with rifampicin resistance not detected by Xpert MTB/RIF

Soedarsono Soedarsono; Ni Made Mertaniasih; Helmia Hasan; Tutik Kusmiati; Ariani Permatasari; Deby Kusumaningrum; Whendy Wijaksono

doi:10.4103/ijmy.ijmy_176_22

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ORIGINAL ARTICLE

Year : 2022 | Volume : 11 | Issue : 4 | Page : 429-434

Line probe assay test in new cases of tuberculosis with rifampicin resistance not detected by Xpert MTB/RIF

Soedarsono Soedarsono¹, Ni Made Mertaniasih², Helmia Hasan³, Tutik Kusmiati⁴, Ariani Permatasari⁴, Deby Kusumaningrum², Whendy Wijaksono³
¹ Department of Pulmonology and Respiratory Medicine, Airlangga University; Sub-Pulmonology Department of Internal Medicine, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia
² Department of Clinical Microbiology, Faculty of Medicine; Tuberculosis Study Group, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
³ Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
⁴ Department of Pulmonology and Respiratory Medicine; Tuberculosis Study Group, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia

Correspondence Address:
Soedarsono Soedarsono
Jl. Mayjen Prof. Dr. Moestopo, No. 47, Surabaya 60131
Indonesia

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/ijmy.ijmy_176_22

Background: In Indonesia, the National guideline for tuberculosis only recommended taking the DST to check INH resistance only for re-treatment cases of rifampicin-susceptible TB (RS-TB) detected by Xpert MTB/RIF. This study was conducted mainly to evaluate the proportion of isoniazid resistance in new cases of RS-TB according to the Xpert MTB/RIF. Methods: This was an observational descriptive study in RS-TB new patients diagnosed by Xpert MTB/RIF. Sputum samples were examined using first-line LPA and evaluated by culture-based DST. Results of first-line LPA and culture-based DST were compared and presented. Results: Fifty-four new cases of RS-TB (according Xpert MTB/RIF) were enrolled in this study. INH resistance was detected in 4 (7.4%) using FL-LPA and in 5 (9.3%) using culture-based DST. RIF resistance was also found in 1 (1.9%) using FL-LPA and in 2 (3.7%) using culture-based DST. Ethambutol resistance was also detected in 4 (7.4%) using culture-based DST. Conclusion: First-line LPA successfully revealed 4 (7.4%) of Hr-TB in new RS-TB cases detected by the Xpert MTB/RIF. In new cases with RS-TB detected by the Xpert MTB/RIF, FL- LPA can be used as rapid molecular DST to detect RIF and INH resistance followed by culture-based DST to examine other drug resistance.

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