The International Journal of Mycobacteriology

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 11  |  Issue : 2  |  Page : 145--149

Investigation of species distribution of nontuberculosis mycobacteria isolated from sputum samples in patients with suspected pulmonary tuberculosis


Serihan Kübra Emikoglu Cerit1, Gülnur Tarhan2, Ismail Ceyhan3 
1 Department of Medical Microbiology, Health Science Institute, Adiyaman University, Adiyaman, Turkey
2 Department of Medical Microbiology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey
3 Department of Public Health Nursing, Health Science Faculty, Ankara Yildirim Beyazit University, Ankara, Turkey

Correspondence Address:
Gülnur Tarhan
Adiyaman Üniversitesi, Tip Fakültesi, Tibbi Mikrobiyoloji Anabilim Dali, Altinsehir Mah. 3005, Sok. No: 13, 02040, Merkez, Adiyaman
Turkey

Aims: Rapid and accurate identification of mycobacteria is important for the species-specific treatment of the disease. The aim of this study was the identification at the species level of 34 nontuberculous mycobacteria strains isolated from respiratory tract samples and 14 reference strains as by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Materials and Methods: Isolates derived from clinical specimens were subcultured in the Lowenstein–Jensen medium. Deoxyribonucleic acid isolation was carried out using the boiling method. PCR amplification was performed using primers specific to the hsp65 gene region. The PCR products were digested BstEII and HaEIII enzymes. All samples were studied comparatively by two different centers. Results: In our study, the most common species were found to be Mycobacterium intracellulare in 23.52% (8/34). The performance of the PCR-RFLP method in detecting mycobacteria was found to be 82.35%. Conclusions: The PCR-RFLP method is a rapid, cheap, and practical method for the identification of mycobacteria.


How to cite this article:
Emikoglu Cerit SK, Tarhan G, Ceyhan I. Investigation of species distribution of nontuberculosis mycobacteria isolated from sputum samples in patients with suspected pulmonary tuberculosis.Int J Mycobacteriol 2022;11:145-149


How to cite this URL:
Emikoglu Cerit SK, Tarhan G, Ceyhan I. Investigation of species distribution of nontuberculosis mycobacteria isolated from sputum samples in patients with suspected pulmonary tuberculosis. Int J Mycobacteriol [serial online] 2022 [cited 2022 Jun 30 ];11:145-149
Available from: https://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=2;spage=145;epage=149;aulast=Emikoglu;type=0