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  Indian J Med Microbiol
 

Figure 2: Formation of hydroxyl radical, H2O2, Fe (II), and superoxide in Mycobacterium smegmatis cultures upon prolonged exposure to moxifloxacin in the absence and presence of TU. (a, c, and h) Line graphs showing the median fluorescence of HPF (for hydroxyl radical), Amplex Red (for H2O2), and CRG (for superoxide), respectively. (b, d, and i) Bar graphs of the quantitation of median fluorescence of HPF, Amplex Red, and CRG for the 30 h, 36 h, or 54 h of the ASP. (e) Line graph showing the Fe (II) levels (μM/cell) determined using FeRhoNox-1 assay. (f) Quantitation of Fe (II) levels for the 30 h and 36 h ASP cells. (g) Quantitation of Fe (II) levels for the 30 h and 36 h (ASP cells) in the presence of 100 μM bipyridyl (iron chelator). The black box shows the peak period of the levels of the fluorescence (due to ROS levels) of HPF, Amplex Red, FeRhoNox-1 (as Fe [II] concentration per cell), and CRG, corresponding to the CFU/ml. Statistical significance was calculated using paired t-test (n = 3 biological replicates in each case). ROS: Reactive oxygen species, HPF: 3'-(p-Hydroxyphenyl) fluorescein CFU: Colony-forming units. CRG: CellRox Green. ASP: Antibiotic-surviving phase, TU: Thiourea, MXF: Moxifloxacin

Figure 2: Formation of hydroxyl radical, H<sub>2</sub>O<sub>2</sub>, Fe (II), and superoxide in <i>Mycobacterium smegmatis</i> cultures upon prolonged exposure to moxifloxacin in the absence and presence of TU. (a, c, and h) Line graphs showing the median fluorescence of HPF (for hydroxyl radical), Amplex Red (for H<sub>2</sub>O<sub>2</sub>), and CRG (for superoxide), respectively. (b, d, and i) Bar graphs of the quantitation of median fluorescence of HPF, Amplex Red, and CRG for the 30 h, 36 h, or 54 h of the ASP. (e) Line graph showing the Fe (II) levels (μM/cell) determined using FeRhoNox-1 assay. (f) Quantitation of Fe (II) levels for the 30 h and 36 h ASP cells. (g) Quantitation of Fe (II) levels for the 30 h and 36 h (ASP cells) in the presence of 100 μM bipyridyl (iron chelator). The black box shows the peak period of the levels of the fluorescence (due to ROS levels) of HPF, Amplex Red, FeRhoNox-1 (as Fe [II] concentration per cell), and CRG, corresponding to the CFU/ml. Statistical significance was calculated using paired <i>t</i>-test (<i>n</i> = 3 biological replicates in each case). ROS: Reactive oxygen species, HPF: 3'-(p-Hydroxyphenyl) fluorescein CFU: Colony-forming units. CRG: CellRox Green. ASP: Antibiotic-surviving phase, TU: Thiourea, MXF: Moxifloxacin