Close
  Indian J Med Microbiol
 

Figure 3: Generation of superoxide, H2O2, and hydroxyl radical, and Fe (II) in the Mycobacterium smegmatis cultures exposed to moxifloxacin for prolonged duration in the presence and absence of DPI. (a, c, and g) Line graphs showing the median fluorescence of CRG, Amplex Red, and HPF, respectively. (b, d, and h) Bar graphs of the quantitation of fluorescence of CRG, Amplex Red, and HPF for the 30 h and 36 h of the ASP during the exposure to moxifloxacin. (e) Line graph showing the Fe (II) levels (μM/cell) determined using FeRhoNox-1 assay. (f) Quantitation of Fe (II) levels for the 30 h and 36 h (ASP cells). The black box shows the peak period of the fluorescence levels corresponding to the CFU/ml. Statistical significance was calculated using paired t-test (n = 3 biological replicates in each case). CRG: CellRox Green, HPF: 3'-(p-Hydroxyphenyl) Fluorescein, CFU: Colony-forming units, DPI: Diphenyleneiodonium chloride, ASP: Antibiotic-surviving phase, Msm: M. smegmatis, MXF: Moxifloxacin, AR: Amplex red, FRN: FeRhoNox-1

Figure 3: Generation of superoxide, H<sub>2</sub>O<sub>2</sub>, and hydroxyl radical, and Fe (II) in the <i>Mycobacterium smegmatis</i> cultures exposed to moxifloxacin for prolonged duration in the presence and absence of DPI. (a, c, and g) Line graphs showing the median fluorescence of CRG, Amplex Red, and HPF, respectively. (b, d, and h) Bar graphs of the quantitation of fluorescence of CRG, Amplex Red, and HPF for the 30 h and 36 h of the ASP during the exposure to moxifloxacin. (e) Line graph showing the Fe (II) levels (μM/cell) determined using FeRhoNox-1 assay. (f) Quantitation of Fe (II) levels for the 30 h and 36 h (ASP cells). The black box shows the peak period of the fluorescence levels corresponding to the CFU/ml. Statistical significance was calculated using paired <i>t</i>-test (<i>n</i> = 3 biological replicates in each case). CRG: CellRox Green, HPF: 3'-(p-Hydroxyphenyl) Fluorescein, CFU: Colony-forming units, DPI: Diphenyleneiodonium chloride, ASP: Antibiotic-surviving phase, <i>Msm</i>: <i>M. smegmatis</i>, MXF: Moxifloxacin, AR: Amplex red, FRN: FeRhoNox-1