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  Indian J Med Microbiol
 

Figure 2: The CFU fold-change of rifampicin resisters of M. smegmatis NCF and SCF exposed to 10× MBC rifampicin for 120 h. (a and b) The fold-change in the CFU of rifampicin resisters of NCF and SCF, respectively, plated at 6-h intervals on rifampicin plates (50× MBC) after exposure to 10× MBC rifampicin. The expected 4-fold increase in CFU in 6 h is indicated by the horizontal dotted line. (c and d) The CFU spurts at specific 6-h intervals of (c) NCF and (d) SCF. The spurts of CFU beyond the expected 4-fold in both the samples in 6-h periods are indicated in red boxes. (e and f) The rifampicin resister generation frequency of: (e) NCF and (f) SCF, calculated from the first time point of emergence of the resister colonies. n = 3 in all cases. MBC: Minimum bactericidal concentration, CFU: Colony forming unit, NCF: Normal-sized cells-enriched fraction, SCF: Short-sized cells-enriched fraction

Figure 2: The CFU fold-change of rifampicin resisters of <i>M. smegmatis</i> NCF and SCF exposed to 10× MBC rifampicin for 120 h. (a and b) The fold-change in the CFU of rifampicin resisters of NCF and SCF, respectively, plated at 6-h intervals on rifampicin plates (50× MBC) after exposure to 10× MBC rifampicin. The expected 4-fold increase in CFU in 6 h is indicated by the horizontal dotted line. (c and d) The CFU spurts at specific 6-h intervals of (c) NCF and (d) SCF. The spurts of CFU beyond the expected 4-fold in both the samples in 6-h periods are indicated in red boxes. (e and f) The rifampicin resister generation frequency of: (e) NCF and (f) SCF, calculated from the first time point of emergence of the resister colonies. <i>n</i> = 3 in all cases. MBC: Minimum bactericidal concentration, CFU: Colony forming unit, NCF: Normal-sized cells-enriched fraction, SCF: Short-sized cells-enriched fraction