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  Indian J Med Microbiol
 

Figure 4: The lineage of consecutive divisions of M. smegmatis NCF and SCF cells from the 10× MBC rifampicin exposed culture. The NCF and SCF cells were sampled for live-cell time-lapse imaging from the 60-h and 84-h of the exposure shown in [Figure 1]a. (a and d) The DIC images of the: (a) NCF and (d) SCF cells taken at different time points. (b and e) The lineage tracked from the live-cell time-lapse images of Supplementary Video S1 (b) on NCF cells and Supplementary Video S2 (e) on SCF cells. The zero-time point does not correlate with the birth of the starting mother cell. Blue arrows indicate multiple constrictions. Red arrows indicate specific divisions. Black-and red-colored lines indicate different sibling-daughter cells. The cell-length was not drawn to scale. (c and f) The division duration of the individual: (c) NCF cells and (f) SCF cells between consecutive divisions, calculated by measuring the time taken from its birth to the next division. One video image each of the three biological replicates of the NCF and SCF subpopulations is shown. NCF: Normal-sized cell-enriched fraction, SCF: Short-sized cell-enriched fraction, DIC: Differential Interference Contrast

Figure 4: The lineage of consecutive divisions of <i>M. smegmatis</i> NCF and SCF cells from the 10× MBC rifampicin exposed culture. The NCF and SCF cells were sampled for live-cell time-lapse imaging from the 60-h and 84-h of the exposure shown in [Figure 1]a. (a and d) The DIC images of the: (a) NCF and (d) SCF cells taken at different time points. (b and e) The lineage tracked from the live-cell time-lapse images of Supplementary Video S1 (b) on NCF cells and Supplementary Video S2 (e) on SCF cells. The zero-time point does not correlate with the birth of the starting mother cell. Blue arrows indicate multiple constrictions. Red arrows indicate specific divisions. Black-and red-colored lines indicate different sibling-daughter cells. The cell-length was not drawn to scale. (c and f) The division duration of the individual: (c) NCF cells and (f) SCF cells between consecutive divisions, calculated by measuring the time taken from its birth to the next division. One video image each of the three biological replicates of the NCF and SCF subpopulations is shown. NCF: Normal-sized cell-enriched fraction, SCF: Short-sized cell-enriched fraction, DIC: Differential Interference Contrast