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  Indian J Med Microbiol
 

Figure 4: Melt peak chart of real-time PCR showing mRNA expression analysis of GAPDH, TLR2, TLR4 and TLR9 in various groups. Note: As shown above in [Figure 4], a single sharp melt peak was observed validating the specificity of PCR product and absence of primer dimmers in the RT-PCR reaction. RT-PCR: Reverse transcription-polymerase chain reaction, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, TLR: Toll-like receptors, PCR: Polymerase chain reaction, mRNA: Messenger RNA

Figure 4: Melt peak chart of real-time PCR showing mRNA expression analysis of GAPDH, TLR2, TLR4 and TLR9 in various groups. Note: As shown above in [Figure 4], a single sharp melt peak was observed validating the specificity of PCR product and absence of primer dimmers in the RT-PCR reaction. RT-PCR: Reverse transcription-polymerase chain reaction, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, TLR: Toll-like receptors, PCR: Polymerase chain reaction, mRNA: Messenger RNA